Used as a substrate in enzymatic assays to study β-glucosidase activity. It acts as a chromogenic compound where enzyme cleavage releases phenylthiol, which can be detected spectrophotometrically. Commonly employed in biochemical research to monitor enzyme kinetics, inhibition, and activation. Also utilized in the development of diagnostic kits for enzyme-related disorders. Its stability and specificity make it suitable for high-throughput screening in drug discovery.