Used in plant physiology and agricultural research to measure the activity of RubisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase), a key enzyme in the photosynthetic process. Helps assess plant health, photosynthetic efficiency, and stress responses under varying environmental conditions such as drought, high temperature, or pollution. Widely applied in studies aiming to improve crop yield and develop climate-resilient plants. Also used in ecological research to compare photosynthetic performance across species or ecosystems. The ELISA format allows high-throughput, sensitive quantification of active enzyme levels in leaf tissue extracts.