Used in biomedical research to measure angiotensin-converting enzyme (ACE) activity in rat samples, particularly in studies related to the renin-angiotensin system. Commonly applied in cardiovascular research to evaluate ACE levels in serum, plasma, or tissue homogenates. Helps assess the effects of drugs or disease models on ACE activity, such as in hypertension, heart failure, or kidney disease. Provides a sensitive and specific method for high-throughput screening in preclinical studies.