Used in biomedical research to measure AMP-activated protein kinase (AMPK) activity in rat tissue samples or cell lysates. This ELISA kit enables researchers to quantify phosphorylated AMPK levels, serving as an indicator of metabolic status in studies related to energy homeostasis, diabetes, obesity, and exercise physiology. It is widely applied in preclinical studies to evaluate drug effects on metabolic pathways and signaling mechanisms in rat models. The assay provides a sensitive and specific method for high-throughput screening in metabolic disease research.