Used in biomedical research to measure hydroxybutyrate dehydrogenase (HBDH) activity in rat samples, particularly in studies related to cardiac and mitochondrial function. The kit enables quantification of enzyme levels in serum, plasma, or tissue homogenates, supporting research on heart injury, metabolic disorders, and oxidative stress in rat models. It provides a sensitive and specific method for high-throughput analysis in preclinical studies.