Used in biomedical research to measure glutaminase (GLS) enzyme activity in rat tissue samples or cell lysates. Helps assess glutamine metabolism in studies related to neurological disorders, kidney function, and cancer. Commonly applied in preclinical studies where rat models are used to investigate metabolic pathways, mitochondrial function, and the effects of pharmaceutical compounds on glutamate production. Enables high-throughput analysis with reliable quantification, supporting research in neurodegeneration, metabolic diseases, and drug development.