Used as a bifunctional crosslinking agent in bioconjugation, particularly for coupling peptides or proteins to surfaces or other biomolecules. The compound contains a succinimide ester and a maleimide group, enabling selective reactions with primary amines and thiol groups, respectively. Commonly applied in the development of immunoassays, antibody-drug conjugates, and functionalized nanoparticles. Its sulfonate group enhances water solubility, making it suitable for use in aqueous reaction environments without organic solvents. Also employed in immobilizing biomolecules on biosensor surfaces due to its stable linkage and controlled orientation.