Used in bioconjugation processes to link proteins or peptides with other molecules such as antibodies, enzymes, or labels. Its primary application is in the development of immunoassays and targeted drug delivery systems. The reagent enables controlled crosslinking through thiol and amine groups, allowing stable yet cleavable bond formation. Commonly applied in preparing protein-protein conjugates for research and diagnostic tools. Water-soluble nature due to sulfo group enhances performance in biological systems without requiring organic solvents.