Used in research settings to study the function of epithelial sodium channels (ENaC), particularly in relation to fluid regulation in the lungs and kidneys. Shows potential as a tool compound for understanding diseases like cystic fibrosis, where ENaC activity is dysregulated. Also investigated for its effects on blood pressure due to ENaC's role in sodium reabsorption in the renal system. Highly selective inhibition allows for precise experimental control in cellular and animal models.