Used in peptide synthesis as a chiral building block for constructing complex peptides with defined stereochemistry. Its orthogonal protecting groups (Boc and FMoc) allow selective deprotection, enabling stepwise assembly of peptide chains. Particularly useful in solid-phase peptide synthesis where controlled coupling and deprotection are critical. The proline scaffold influences peptide conformation, often promoting specific secondary structures like turns or helices, making it valuable in drug design and development of bioactive peptides.