Used for reversible biotinylation of proteins and cell surface labeling. Enables isolation of biotinylated molecules via streptavidin affinity purification. The disulfide bond allows cleavage under reducing conditions, making it ideal for capturing and releasing protein complexes in pull-down assays. Commonly applied in proteomics to study protein-protein interactions. Suitable for labeling primary amines on live cells with minimal toxicity, followed by cell lysis and target recovery. Also used in crosslinking experiments where temporary tagging is required.