TBE Buffer is widely used in molecular biology for electrophoresis of nucleic acids, particularly DNA and RNA. It provides a stable pH environment that helps maintain the integrity of nucleic acids during gel electrophoresis. The buffer supports efficient separation of DNA fragments by size in agarose and polyacrylamide gels. TBE has good buffering capacity due to its components, which help conduct electric current and prevent pH shifts during the run. It is preferred in high-resolution applications such as analyzing small DNA fragments or in sequencing gels because it offers better resolution compared to some other buffers. Additionally, TBE is compatible with downstream applications like DNA extraction from gels or enzymatic reactions, making it a standard choice in laboratories for routine and advanced nucleic acid analysis.