Used primarily as a photoactivatable crosslinking agent in biochemical and proteomic research. Its acrylate group enables conjugation to biomolecules, while the trifluoromethyl diazirine moiety generates highly reactive carbenes upon UV irradiation. These carbenes insert into nearby C–H, N–H, or O–H bonds, forming covalent links between interacting biomolecules such as proteins or protein-nucleic acid complexes. This allows researchers to capture transient or weak molecular interactions in situ, making it valuable for studying protein-protein interactions, mapping binding sites, and identifying unknown interaction partners in complex biological systems. Commonly employed in pull-down assays, crosslinking mass spectrometry (XL-MS), and photoaffinity labeling experiments.