Used primarily as a photoactivatable crosslinking agent in biochemical and molecular biology research. Its main application lies in photoaffinity labeling, where it helps identify and map interactions between biomolecules such as proteins, nucleic acids, and ligands. Upon exposure to UV light, the diazirine group generates highly reactive carbenes that form covalent bonds with nearby molecules, capturing transient or weak interactions that are difficult to study with other methods. The aniline moiety allows for further chemical modification, enabling conjugation to probes, tags, or functional groups for detection or purification. It is especially valuable in studying protein-protein interactions, receptor-ligand binding, and mapping binding sites in complex biological systems.