Used in environmental toxicology and pharmaceutical research to measure acetylcholinesterase (AChE) activity in zebrafish, serving as a biomarker for neurotoxicity. Helps assess the impact of pesticides, heavy metals, and emerging contaminants on the nervous system. Widely applied in high-throughput screening of drug candidates and ecotoxicological studies due to zebrafish’s genetic similarity to humans and rapid development. Enables quantitative analysis of AChE inhibition, providing insights into mechanisms of neurotoxicity and potential therapeutic interventions.