Used primarily in research settings as a selective inhibitor of Jumonji histone demethylases, particularly targeting the JARID1 family. This activity makes it valuable for studying epigenetic regulation of gene expression, especially in cancer biology where histone methylation plays a key role in tumor progression. It has shown potential in enhancing the efficacy of other anticancer agents by reactivating silenced tumor suppressor genes. Additionally, Z-JIB-04 is employed to investigate cellular differentiation, stem cell reprogramming, and chromatin dynamics due to its ability to modulate the epigenome. Its use remains confined to preclinical studies, supporting drug discovery and mechanistic exploration in epigenetics.