Z-JIB-04 is primarily used as a selective inhibitor of Jumonji histone demethylases, particularly targeting the JARID1 and JMJD2 subfamilies. This activity makes it a valuable tool in epigenetic research, where it helps modulate gene expression by preventing the removal of methyl groups from histone proteins. It has been applied in studies related to cancer biology, as inhibition of histone demethylases can reactivate tumor suppressor genes silenced in various malignancies. Z-JIB-04 also shows potential in enhancing the efficacy of other anticancer agents by promoting differentiation and reducing the self-renewal capacity of cancer stem cells. Additionally, it has been explored in cellular reprogramming and stem cell research due to its ability to influence epigenetic states critical for cell fate decisions.