Used in solid-phase oligonucleotide synthesis, this compound enables the incorporation of zebularine, a cytidine analog, into DNA strands. Its primary application is in research involving epigenetic regulation, where zebularine acts as a DNA methyltransferase inhibitor. By integrating into oligonucleotides, it helps study DNA methylation patterns and gene expression control. It is also utilized in developing modified nucleic acids for therapeutic and diagnostic probes, particularly in cancer research and molecular biology tools. The phosphoramidite group allows for efficient and automated synthesis, making it valuable in producing customized sequences for functional genomics and epigenetics.