Used as a fluorogenic substrate in enzymatic assays, particularly for detecting the activity of proteases such as trypsin-like enzymes and caspases. Upon cleavage by the target enzyme, it releases a fluorescent 7-amino-4-methylcoumarin group, allowing real-time monitoring of enzyme activity. Commonly applied in high-throughput screening, apoptosis research, and drug development where sensitive and quantitative measurement of protease activity is required. Its high specificity for certain serine proteases makes it valuable in biochemical and biomedical research settings.