Used as a fluorogenic substrate in enzymatic assays, particularly for studying protease activity. The compound is cleaved by specific enzymes, releasing the fluorescent group AMC (7-amino-4-methylcoumarin), which can be easily detected under UV light. This makes it valuable in monitoring enzyme kinetics and inhibition in research related to proteolytic enzymes, such as those involved in cellular regulation and disease processes. Commonly applied in assays for caspases and other hydrolases in biochemical and pharmaceutical studies.