Used in clinical diagnostics to assess organ damage, particularly heart and liver conditions, by measuring enzyme activity in blood samples. Plays a key role in cellular metabolism assays, especially in research involving the Krebs cycle and energy production. Employed in biosensors and enzymatic test kits for detecting malate or oxaloacetate in food and beverage industries, such as wine and fruit juice analysis. Also utilized in biochemical research to regenerate cofactors like NADH for coupled enzyme reactions.