Oxalate oxidase is primarily used in biosensors for the detection of oxalate levels in biological samples such as blood and urine. It enables rapid and selective measurement of oxalate, which is critical in diagnosing and monitoring kidney stone diseases and primary hyperoxaluria. The enzyme is also employed in industrial applications, including the development of enzymatic treatments to reduce oxalate content in food products and beverages, improving their safety and nutritional profile. In agriculture, oxalate oxidase has been explored in genetically modified crops to enhance resistance against pathogens that produce oxalic acid, thereby improving plant defense mechanisms. Additionally, it serves as a tool in biochemical research for studying oxalate metabolism and its role in various physiological and pathological processes.