Used in biomedical research to measure cyclic adenosine monophosphate (cAMP) levels in cell or tissue samples. Helps study cellular signaling pathways, especially those involving hormones and neurotransmitters. Commonly applied in endocrinology, neuroscience, and immunology research to understand G-protein coupled receptor (GPCR) activity. Enables high-throughput screening of drug effects on intracellular cAMP production. Provides a sensitive and specific method for quantifying cAMP without requiring radioisotopes. Widely used in academic labs, pharmaceutical development, and diagnostic research.