Used in research settings to quantify levels of human alpha-1 antitrypsin (AAT) in biological samples such as serum, plasma, or cell culture supernatants. This ELISA kit enables detection and measurement of AAT protein concentrations, supporting studies related to inflammatory diseases, lung disorders like emphysema, and liver conditions associated with AAT deficiency. It is also utilized in monitoring therapeutic efficacy in patients receiving AAT replacement therapy and in investigating the role of AAT as an acute-phase protein during infection or tissue injury. The assay provides a sensitive and specific tool for clinical and experimental investigations involving immune response and protease-antiprotease balance.