Used in biomedical research to detect and quantify levels of Caspase-9 protein in cell lysates or tissue samples. Plays a critical role in studies related to apoptosis, particularly in understanding the intrinsic pathway of programmed cell death. Commonly applied in cancer research to evaluate the activation of Caspase-9 in response to chemotherapeutic agents or cellular stress. Also utilized in neurodegenerative disease studies, where dysregulation of apoptosis contributes to neuronal loss. Supports high-throughput screening of drug candidates that modulate apoptotic signaling. Provides a reliable, sensitive, and specific method for monitoring Caspase-9 activity without requiring radioactive labels or complex instrumentation.