Used in biochemical research for the oxidation of cholesterol to cholest-5-en-3-one, this enzyme serves as a key catalyst in steroid transformation studies. It is commonly employed in diagnostic kits to measure cholesterol levels in clinical samples by coupling the reaction with a detectable signal system. The lyophilized form ensures stability and ease of storage, making it suitable for use in enzyme-linked assays and industrial-scale biotransformations. Its specificity for cholesterol allows accurate quantification in serum and other biological fluids.