Used in biomedical research to detect and quantify deubiquitinating enzyme (DUB) activity in cell lysates or tissue samples. Helps study protein degradation pathways, ubiquitin signaling, and proteasome function. Widely applied in cancer research, neurodegenerative disease studies (like Parkinson's and Alzheimer's), and drug development targeting ubiquitin-related pathways. Enables high-throughput screening of DUB inhibitors or activators in experimental therapeutics. Provides a reliable, sensitive method for monitoring changes in DUB levels under different physiological or pathological conditions.