Used in research laboratories to measure 25-hydroxyvitamin D levels in human serum or plasma samples. This ELISA kit enables quantitative analysis of vitamin D status, supporting studies on nutritional health, bone disorders, immune function, and metabolic diseases. It is suitable for clinical research and epidemiological studies where accurate assessment of vitamin D deficiency or sufficiency is required. The assay is designed for in vitro use only and provides a reliable, high-throughput method for detecting total 25(OH)D concentrations with good sensitivity and specificity.