Used in medical and pharmaceutical research for its role in studying cell membrane dynamics and endocytosis. It serves as a tool to investigate intracellular trafficking due to its ability to interfere with vesicle formation in eukaryotic cells. Commonly applied in neuroscience to examine synaptic vesicle recycling and neurotransmitter release. Also utilized in the development of targeted drug delivery systems by mimicking natural endocytic pathways. Its high specificity makes it valuable for probing clathrin-mediated internalization processes in cell culture models.