Used in biomedical research to quantify Prostaglandin E2 levels in biological samples such as serum, plasma, and cell culture supernatants. Widely applied in immunology and reproductive studies due to PGE2's role in inflammation, immune regulation, and parturition. Enables researchers to monitor changes in PGE2 concentration during experimental treatments, disease models, or physiological conditions. Particularly valuable in studies involving hormonal regulation, inflammatory diseases, and cancer progression. The ELISA format allows for high sensitivity, specificity, and throughput in detecting low concentrations of PGE2.