Used in research settings to detect and quantify levels of human ubiquitin ligase (E3/UBPL) in biological samples such as cell lysates or tissue extracts. This kit enables scientists to study the regulation of protein degradation via the ubiquitin-proteasome system, which plays a critical role in cellular processes including cell cycle control, DNA repair, and apoptosis. It is commonly applied in cancer research, neurodegenerative disease studies, and drug development where dysregulation of ubiquitin ligase activity is implicated. The assay provides a reliable, sensitive, and high-throughput method for monitoring E3 ligase expression and activity under experimental conditions.