Used in research settings to quantify ubiquitin binding enzyme (E2) activity in biological samples. Helps investigate protein ubiquitination pathways involved in cellular regulation, DNA repair, cell cycle control, and degradation of damaged or misfolded proteins. Widely applied in studies related to neurodegenerative diseases, cancer, and autoimmune disorders where ubiquitin-proteasome system dysfunction occurs. Enables high-throughput screening of potential therapeutic compounds that modulate E2 enzyme activity. Provides a reliable, sensitive, and specific method for monitoring changes in E2/UBCE levels in cell lysates or tissue extracts under experimental conditions.