Used to detect and quantify caspase-3 activity in biological samples such as cell lysates, tissue homogenates, and serum. Plays a critical role in apoptosis research, helping scientists study programmed cell death in diseases like cancer, neurodegenerative disorders, and autoimmune conditions. Enables high-throughput screening of drug candidates that modulate apoptotic pathways. Widely used in preclinical studies to evaluate treatment-induced cell death and assess the efficacy of therapeutic agents. Provides a reliable, sensitive, and specific method for monitoring caspase-3 activation, a key marker of apoptosis execution.