Used in biochemical and medical research to quantify butyrate kinase enzyme levels in rat samples, aiding studies on gut microbiota metabolism, short-chain fatty acid production, and intestinal health. Commonly applied in preclinical investigations related to inflammatory bowel disease, metabolic disorders, and the role of butyrate in cellular energy regulation and gene expression. Enables high-throughput analysis in laboratory settings for accurate measurement of enzyme concentration in biological fluids or tissue extracts.