Used in research settings to measure levels of 6-keto-PGF1α, a stable metabolite of prostacyclin, in biological samples such as plasma, serum, or tissue homogenates. This assay helps assess vascular endothelial function and platelet activity, as prostacyclin plays a key role in inhibiting platelet aggregation and promoting vasodilation. Commonly applied in studies related to cardiovascular diseases, inflammation, thrombosis, and oxidative stress. The kit enables high-throughput, quantitative analysis, supporting both clinical research and preclinical studies investigating endothelial health and the effects of pharmacological agents on prostaglandin pathways.