Used in biomedical research to quantitatively measure fructose 1,6-diphosphate aldolase levels in human serum, plasma, or tissue homogenates. This assay helps investigate metabolic disorders related to glycolysis and gluconeogenesis. It is also applied in studies of liver function, muscle damage, and certain cancers where aldolase expression may be altered. The kit supports high-throughput screening in clinical labs and research settings due to its sensitivity and specificity.