Used in biomedical research to measure glucocorticoid hormone levels in mouse samples such as serum, plasma, or tissue homogenates. Helps in studying stress responses, immune regulation, and adrenal function in experimental models. Widely applied in endocrinology, neuroscience, and immunology studies to assess HPA axis activity and the effects of drugs or environmental factors on hormone levels. Provides a sensitive and specific method for high-throughput screening in preclinical research.