Used in biomedical research to quantify levels of Growth Differentiation Factor 5 (GDF-5) in biological samples such as serum, plasma, and cell culture supernatants. Enables researchers to study GDF-5's role in bone and cartilage development, joint formation, and tissue repair. Commonly applied in osteoarthritis studies, regenerative medicine, and stem cell differentiation research. Provides a reliable, sensitive, and specific method for monitoring GDF-5 expression in experimental models, supporting drug development and evaluation of therapeutic interventions targeting musculoskeletal disorders.