Used in biomedical research to quantify levels of GAPDH protein in cell lysates, tissue extracts, and biological samples. Helps assess cellular metabolic activity, as GAPDH is a key enzyme in glycolysis. Commonly applied in studies related to cancer metabolism, neurodegenerative diseases, and diabetes where glycolytic flux is altered. Also used as a loading control in protein expression studies to normalize data across samples. Enables high-throughput analysis in microplate format, supporting reproducible and sensitive detection of GAPDH under various experimental conditions.