Used in biomedical research to detect and quantify β-galactosidase enzyme activity in cell lysates or tissue samples. Commonly applied in studies involving gene expression, particularly in reporter gene assays where β-galactosidase serves as a marker. Also utilized in aging research, as β-galactosidase activity at pH 6.0 is a biomarker for senescent cells. Enables high-throughput screening in drug development and evaluation of cellular senescence in disease models such as cancer, fibrosis, and age-related conditions. Provides a sensitive and specific method for monitoring transfection efficiency and promoter activity in molecular biology experiments.