Used in biochemical and endocrinology research to measure levels of 17β-hydroxysteroid dehydrogenase in fish samples. This enzyme plays a key role in steroid metabolism, particularly in the conversion of sex hormones like testosterone and estradiol. The ELISA kit enables researchers to quantify enzyme activity in tissue extracts or plasma, supporting studies on fish reproduction, sexual development, and endocrine disruption caused by environmental pollutants. It is widely applied in aquaculture research, toxicology testing, and ecological monitoring to assess hormonal health and the impact of endocrine-disrupting chemicals in aquatic organisms.