Used in biomedical research to quantify levels of heat shock protein 27 in cell lysates, tissue extracts, and biological fluids such as serum or plasma. Enables researchers to study cellular stress responses, apoptosis regulation, and inflammatory processes. Commonly applied in studies related to neurodegenerative diseases, cardiovascular conditions, cancer progression, and autoimmune disorders. Supports evaluation of HSP-27 as a potential biomarker in clinical and preclinical research settings.