Used in biomedical research to quantify heparan sulfate levels in biological samples such as serum, plasma, and cell culture supernatants. Enables investigation of heparan sulfate involvement in processes like inflammation, viral entry (including SARS-CoV-2), cancer progression, and extracellular matrix remodeling. Commonly applied in studies related to kidney disease, coagulation disorders, and developmental biology. Provides a reliable, high-throughput method for detecting HS concentration changes in experimental models, supporting both basic science and translational research.