Used in molecular biology for DNA digestion, particularly in restriction analysis and epigenetic studies. Cleaves DNA at specific recognition sites (5'-CCGG-3') and is commonly applied in methylation-sensitive PCR, genomic mapping, and bisulfite sequencing validation. Its high specificity allows differentiation between methylated and unmethylated DNA regions, making it valuable in gene expression studies, cancer research, and epigenetic regulation analysis. Often used in combination with other enzymes in double digest protocols to confirm DNA modification patterns.