Used in veterinary research to measure histamine levels in canine biological samples such as serum, plasma, or tissue homogenates. Helps in studying allergic reactions, inflammatory responses, and mast cell activity in dogs. Commonly applied in immunology and dermatology studies to evaluate the severity of allergic conditions and monitor the effectiveness of treatments. Provides a sensitive and specific method for quantifying histamine, supporting research into canine atopic dermatitis, food allergies, and anaphylactic responses.