Used in biochemical and medical research to measure levels of hydroxybutyrate dehydrogenase enzyme in rat samples. Helps assess cardiac and mitochondrial function, as HBDH activity correlates with tissue damage, especially in heart muscle. Commonly applied in studies of myocardial infarction, metabolic disorders, and drug toxicity in rodent models. Enables quantitative analysis of enzyme concentration in serum or tissue homogenates, supporting preclinical research and evaluation of therapeutic interventions.