Used in biomedical research to quantify α-hydroxybutyrate dehydrogenase levels in rat serum or tissue samples. Helps assess myocardial or cellular damage, particularly in studies related to heart injury, ischemia, and metabolic disorders. Commonly applied in preclinical trials, toxicology studies, and disease models involving oxidative stress or organ dysfunction. Provides a reliable, sensitive method for enzyme activity measurement through immunoassay techniques.