Used in biomedical research to measure serotonin levels in rat samples, this ELISA kit enables accurate quantification of 5-hydroxytryptamine (5-HT) in serum, plasma, or tissue homogenates. It is widely applied in neuroscience studies to investigate mood disorders, sleep, appetite, and neurochemical changes in response to drugs or experimental treatments. The assay supports high-throughput analysis with high sensitivity and specificity, making it ideal for preclinical studies assessing the function of the serotonergic system. Researchers also use it in toxicology and pharmacology to evaluate the impact of compounds on serotonin metabolism in animal models.