Used in biomedical research to measure levels of 11β-hydroxysteroid dehydrogenase type 2 enzyme in rat tissue samples, particularly in studies related to steroid metabolism, hypertension, and kidney function. Helps researchers assess enzyme activity in adrenal and renal tissues where it plays a key role in protecting mineralocorticoid receptors by inactivating cortisol. Commonly applied in endocrine studies, toxicology research, and experiments investigating the renin-angiotensin-aldosterone system. Provides a reliable, sensitive method for quantifying protein expression in experimental models of metabolic and cardiovascular diseases.