Used in biomedical research to detect and quantify levels of troponin I specifically from rat skeletal muscle in biological samples such as serum or tissue extracts. This assay is commonly applied in studies related to muscle injury, myopathy, or exercise physiology in rat models. It helps researchers assess skeletal muscle damage and regeneration, and differentiate it from cardiac injury, due to its specificity for the skeletal muscle isoform. The kit is not intended for diagnostic use but supports preclinical research in pharmacology, toxicology, and muscle disease modeling.